-
Product Name
Mouse E-Cadherin/CDH1/E-cad/CD324 (His Tag) recombinant protein
- Documents
-
Description
Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7 (By similarity).
-
Protein name
Cadherin-1
-
Protein short names
L-CAM; CDHE; ECAD; UM; LCAM; E-CADHERIN; CD324; MGC107495; AA960649; E-CAD; ARC-1; CDH1; UVO
-
Uniprot ID
P09803
-
Gene Name
Cdh1
-
Source/Expression Host
Human Cells
-
Expression Plasmid/cDNA
A DNA sequence encoding the extracellular domain of mouse CDH1 (P09803) (Met 1-Val 709) was expressed, with a C-terminal polyhistidine tag.
-
Protein Species
Mouse
-
Molecular weight
The secreted recombinant mouse CDH1 (pro form) comprises 697 amino acids and has a calculated molecular mass of 78 kDa. As a result of different glycosylation, the apparent molecular mass of rm CDH1 is approximately 80-100 kDa in SDS-PAGE under reducing conditions.
-
Purity
> 85 % as determined by SDS-PAGE
-
Activity
Measured by the ability of the immobilized protein to support the adhesion of MCF-7 human breast adenocarcinoma cells. When 5 x 10E4 cells/well are added to Recombinant Mouse Cadherin-1 coated plates (0.8 μg/mL with 100 μL/well), approximately >50% will adhere after 1 hour at 37℃.
-
Validations
Mouse E-Cadherin / CDH1 / E-cad / CD324 Protein (His Tag) SDS-PAGE
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"