Anti-E Cadherin (7H12) Mouse antibody

Cat.#: 168486

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Product Information

  • Product Name
    Anti-E Cadherin (7H12) Mouse antibody
  • Documents
  • Description
    E Cadherin (7H12) Mouse monoclonal antibody
  • Tested applications
    WB, IHC-P, FC
  • Species reactivity
    Human, Mouse, Monkey
  • Isotype
    Mouse IgG1
  • Preparation
    Antigen: Purified recombinant fragment of human CDH1 expressed in E. Coli.
  • Clonality
    Monoclonal
  • Formulation
    Ascitic fluid containing 0.03% sodium azide.
  • Storage instructions
    Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Applications

    WB: 1/500 - 1/2000

    IHC: 1/200 - 1/1000

    FC: 1/200 - 1/400

    ELISA: 1/10000

  • Validations

    Western blot analysis of extract from T47D cells using E-Cadherin Mouse mAb at 1:1000 dilution. Predicted band size: 135KDa. Observed band size: 135KDa.

    Western blot analysis of extract from T47D cells using E-Cadherin Mouse mAb at 1:1000 dilution. Predicted band size: 135KDa. Observed band size: 135KDa.

    Western blot detection of E-Cadherin in C6 and HaCat cell lysates using E Cadherin mouse mAb (dilution 1:500).Predicted band size:135KDa.Observed band size:135KDa.

    Western blot detection of E-Cadherin in C6 and HaCat cell lysates using E Cadherin mouse mAb (dilution 1:500).Predicted band size:135KDa.Observed band size:135KDa.

  • Background
    Swiss-Prot Acc.P12830.E-Cadherin is a 120 kDa transmembrane glycoprotein that is localized in the adherens junctions of epithelial cells. There, it interacts with the cytoskeleton through the associated cytoplasmic catenin proteins. In addition to being a calcium-dependent adhesion molecule, E-Cadherin is also a critical regulator of epithelial junction formation. Its association with catenins is necessary for cell-cell adhesion. These E-cadherin/catenin complexes associate with corical actin bundles at both the zonula adherens and the lateral adhesion plaques. Tyrosine phosphorylation can disrupt these complexes, leading to changes in cell adhesion properties. E-Cadherin expression is often down-regulated in highly invasive, poorly differentiated carcinomas. Increased expression of E-Cadherin in these cells reduces invasiveness. Thus, loss of expression or function of E-Cadherin appears to be an important step in tumorigenic progression.Tissue specificity: Non-neural epithelial tissues.
  • References

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"