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Product Name
Human E-Cadherin/CDH1/E-cad/CD324 (Fc Tag) recombinant protein
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Description
Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.
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Protein name
Cadherin-1
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Protein short names
MGC107495; CD324; E-CADHERIN; LCAM; UM; ECAD; CDHE; L-CAM; UVO; CDH1; ARC-1; E-CAD; AA960649
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Uniprot ID
P12830
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Gene Name
CDH1; CDHE; UVO
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Source/Expression Host
Human Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the human E-Cad (P12830)(Met1-Ile707) was expressed with the Fc region of human IgG1 at the C-terminus.
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Protein Species
Human
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Molecular weight
The recombinant human E-Cad/Fc is a disulfide-linked homodimer. The reduced monomer comprises 791 amino acids and has a predicted molecular mass of 87.1 kDa. The apparent molecular mass of the protein is approximately 116-126 kDa in SDS-PAGE under reducing conditions.
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Purity
> 90 % as determined by SDS-PAGE
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Activity
Measured by the ability of the immobilized protein to support the adhesion of MCF-7 human breast adenocarcinoma cells.
When cells are added to E-Cad coated plates (5 μg/mL, 100 μL/well), approximately 33% will adhere specifically after 90 minutes at 37 ℃. -
Validations
Human E-Cadherin / CDH1 / E-cad / CD324 Protein (Fc Tag) SDS-PAGE
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