Loading controls are used to ensure equal loading of a gel and are important when western blot results are being compared qualitatively or quantitatively. Loading controls often used are proteins which express well across many cell lines and tissues, these include targets such as: β-Actin, COX IV, GAPDH, Histone H3, and α- and β-Tubulin.
We offer antibodies against the most common loading and expression control proteins. Loading control antibodies are helpful for assessing western blotting efficiencies and comparing the amounts of protein loaded in each well across a gel. These controls help determine whether sample-to-sample expression level differences are due to actual protein levels in a given cell lysate or from loading variances. Loading control antibodies can also be used as complementary antibody stains in immunofluorescence studies with proteins of interest. Loading control antibodies are available in unconjugated form, or conjugated to biotin, HRP etc.