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Product Name
Anti-E cadherin Rabbit antibody
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Description
E cadherin Rabbit polyclonal antibody
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Tested applications
WB, IHC-P, ICC/IF, ELISA
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Species reactivity
Human, Mouse, Rat
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Isotype
Rabbit IgG
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Preparation
Antigen: The antiserum was produced against synthesized peptide derived from human Cadherin. AA range:833-882
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Clonality
Polyclonal
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Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/500 - 1/2000
IHC: 1/100 - 1/300
IF: 1/200 - 1/1000
ELISA: 1/20000
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Validations
Western blot analysis of extract from T47D cells using E-Cadherin Rabbit pAb at 1:1000 dilution. Predicted band size: 135kDa. Observed band size: 135kDa.
Western blot detection of E-Cadherin in Jurkat,3T3,COS7 and C6 cell lysates using E-Cadherin rabbit pAb (1:1000 diluted).Predicted band size:135kDa.Observed band size:135kDa.
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Background
Swiss-Prot Acc.P12830.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"