Terrific Broth (TB) preparation
Terrific Broth (TB)12 g/L tryptone (1.2% m/V)24 g/L yeast extract (2.4% m/V)4 mL/L glycerol (4.34 mM, 0.4% V/V)17 mM KH2PO4, 2.31 g/L72 mM K2HPO4, 12.54 g/L(opt.) 15 g/L agar (1.5% m/V)(opt., nontrad.) 5–10 mM 1 M MgSO4 or MgCl2The original sug...
View More