Green Indicator Plates
Jeff Lawrence (Z51)(NOTE: This section describes classic green plates, which have now been superceded by "Mint Green Plates")Plate Mix 828 g Bacto-Tryptone (Difco) 105 g Yeast Extract (Difco) 516 g NaCl 1551&nb...
View MoreJeff Lawrence (Z51)(NOTE: This section describes classic green plates, which have now been superceded by "Mint Green Plates")Plate Mix 828 g Bacto-Tryptone (Difco) 105 g Yeast Extract (Difco) 516 g NaCl 1551&nb...
View MoreProtocolModification of Jeff Lawrence (X10)1. Prepare media in two flasks: Flask A 12 g Agar 4 g Tryptone 4 g Yeast Extract 40 mg Anhydrotetracycline 400 mL Water Flask B 8 g NaCl 8 g NaH2PO4 40...
View MoreAmino acid Stock Concentration Molecular Weight gm/100ml ml/liter ml/plate Arginine 50mM 100mM 210.7 1.054 2.107 10 5 0.2 0.1 Asparagine 300 mM 132.1 7.926 10 0.2 Histidine HCl 100 mM 209 2.09 3 0.1 Isoleucine 50 mM 131.2 0.656 10 0.2 Leucine 100 mM...
View MoreJeff Lawrence (Z50)50X E Salts1. Heat 1 L distilled water in a 4 liter beaker.2. Dissolve completely, in indicated order: 300 g citric acid monohydrate (H3C6H5O7·H2O) 14.1 g MgSO4 1965 g K2HPO4·3H2O 525 ...
View MoreAntibiotic AbbreviationConcentration1Concentration1Stock Solution2 (Rich Media)(Minimal Media)ml/liter Na Ampicillin3 Amp, Ap 50 μg/ml 15 μg/ml4 20 mg/mL 50% EtOH Chloramphenicol Cam, Cm 20 μg/...
View MoreSubstancepKadpKa/dTMrOxalate (oxalic acid) (pK1)1.2790.0Maleate (maleic acid) (pK1)2.000116.1Phosphate (phosphoric acid) (pK1)2.150.004498.0Glycine (pK1)2.35−0.00275.1Phthalate (phthalic acid) (pK1)2.950.0020166.1Glycylglycine (pK1)3.12132.1Malate (m...
View MoreTo predict transcription factor binding site in DNA, you may use the following tools:1. PROMOPROMO is a virtual laboratory for the identification of putative transcription factor binding sites (TFBS) in DNA sequences from a species or groups of speci...
View MoreThe common perception of scaling of dose based on the body weight (mg/kg) alone is not the right approach. This is primarily because the biochemical, functional systems in species vary which in turn alter pharmacokinetics. Therefore, extrapolation of...
View MoreCell adhesion is a pH-dependent phenomenon: the cells required Mg2+ or Ca2+ for attachment to substrates at pH higher than the isoelectric point of the proteins coating a plastic surface, but not at lower pH. With increasing pH from 3.4, cell adhesiv...
View MoreKnowledge and improved understanding of the properties of enzyme active sites and their assorted catalytic mechanisms is vital for novel protein design and predicting protein function from structure. Crystallographic and NMR studies of enzymes have ...
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