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Introduction Because the human body cannot synthesize or synthesize very little and must be obtained from food, linoleic acid is called an essential fatty acid. And because linoleic acid can lower blood cholesterol and prevent atherosclerosis, it has...

Introduction: For a long time, insulin resistance and elevated blood glucose levels are thought to be the cause of type 2 diabetes. However, in a recently published study, the evidence provided by scientists shows that facts may be very differen...

Sodium azide can interfere during the conjugation reaction. We recommend a maximum concentration of 0.1% sodium azide. Nevertheless, sodium azide is known as an irreversible inhibitor of HRP. We do not recommend performing a HRP conjugation in presen...

For long term storage at 4°C we recommend adding antimicrobial agents and/or stabilizers (e.g. azide, BSA, glycerol, etc.). A new conjugate can be stored for 12-18 months at 4°C as long as the antibody will tolerate storage at 4°C. As the bond betwee...

If you are interested in trying to recover functional protein (as suggested by your interest in Native-PAGE), then you are far better off not using an SDS gel in the first place. Bio-Rad makes an apparatus specifically for running native tube gels an...

If you cloned your gene via restriction sites, then you can cleave your ligated vector with the respective enzymes and release your insert. This insert should have the correct size. Additionally, you can cleave with two restriction enzymes, one ...

Introduction: Aspirin is one of the three most classic drugs in the history of medicine. Recently, this "magic drug" has entered the top medical journal New England Journal of Medicine. This time, what has it achieved?On February 22, in the...

Proper pH of fixative and good mounting media is the essential for a good signal to noise ratio. You may want to make up fresh 4% (or less) PFA, which can be stored at 4 degrees Celsius for a week or so.For a 4% PFA solution follow this protocol.Add ...

Add 1/10 vol of NH4OAc, en 2.5 volume 100% cold EtOH (100%) and 1 µl glycogen (if you think you'll the pellet without see glycogen you can do without it)mixIncubate 30 min at -80°CCentrifuge 20 min at 12000g at 4°C(remove supernatant)Wash with 75...

The pH dependence is usually due to the side groups of the amino acids. A change in pH changes the protonation pattern and can, in extreme cases, result in protein denaturation. I therefore suspect that your protein stabilizes the enzyme structure, t...