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Taking Omega-6 Fatty Acids may prevent premature death

Introduction Because the human body cannot synthesize or synthesize very little and must be obtained from food, linoleic acid is called an essential fatty acid. And because linoleic acid can lower blood cholesterol and prevent atherosclerosis, it has...

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How to store the conjugated antibody?

For long term storage at 4°C we recommend adding antimicrobial agents and/or stabilizers (e.g. azide, BSA, glycerol, etc.). A new conjugate can be stored for 12-18 months at 4°C as long as the antibody will tolerate storage at 4°C. As the bond betwee...

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How to confirm an insert in a plasmid without sequencing?

If you cloned your gene via restriction sites, then you can cleave your ligated vector with the respective enzymes and release your insert. This insert should have the correct size. Additionally, you can cleave with two restriction enzymes, one ...

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Why is enzymatic activity usually pH dependent?

The pH dependence is usually due to the side groups of the amino acids. A change in pH changes the protonation pattern and can, in extreme cases, result in protein denaturation. I therefore suspect that your protein stabilizes the enzyme structure, t...

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