New Articles List

Introduction: According to the Centers for Disease Control and Prevention, 4% to 6% of children in the United States are allergic to food. The mechanism of food allergy in infants and children has long been a mystery. A new Northwest Medical Res...

Here is a starting point: the Science Signaling journal have a definition here:http://stke.sciencemag.org/about/help/cm.dtl"Canonical Pathways" -- idealized or generalized pathways that represent common properties of a particular signaling ...

You can overlay a biplot of species correlations with the CAP axes. If you are using the PERMANOVA+ software, you can choose to use Spearman or Pearson correlations and set the level of correlation (default is 0.4). Alternatively you can manually cho...

Introduction: A high-fat diet was once considered one of the risks of cardiovascular disease, but scientists do not know the underlying mechanism. Now, the latest research from the University of Augusta Georgia Medical School (MCG) reveals the link b...

Appropriate statistics are Mann-whitney U test and Chi sqaure test.Sometime people use  the t-test to compare responses on likert scale but it is not appropratieBoth the t-test and the MWW can be used.  See: https://www.researchga...

This reduces the chance of heterophilic antibody interference. In most antibody assays, protein is used to prevent these interactions. Heterophilic antibody interference causes both false positives and false negatives.Also, if you just put 10mL of FB...

Introduction: On March 27th, Scientific Journals published an interesting article revealing a structure that has long been missed by traditional anatomy - the interstitium. Because it is widely present throughout the body and involved in many kinds o...

Introduction: Adequate sleep is essential for physical and mental health. Most people need about 8-8.5 hours of sleep every night to feel good. However, scientists have discovered that a genetic mutation can make people sleep only about 5 hours a day...

Common Epitope Tags and SequencesTagProtein SequenceFLAGDYKDDDDK3 x FLAGDYKDHDGDYKDHDIDYKDDDKHAYPYDVPDYAHisHHHHHHHSVQPELAPEDPEDMycEQKLISEEDLV5GKPIPNPLLGLDSTXpressDLDDDDK or DLYDDDDKThrombinLVPRGSBAD (Biotin Acceptor Domain)GLNDIFEAQKIEWHEFactor XaIEG...

Both should work fine, but we always recommend streaking onto agar plates first. If your stocks are contaminated this will often be visible on the plates but very hard to see in broth. Furthermore, if you are counting on doing growth assay and compar...