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This reduces the chance of heterophilic antibody interference. In most antibody assays, protein is used to prevent these interactions. Heterophilic antibody interference causes both false positives and false negatives.Also, if you just put 10mL of FB...

Introduction: On March 27th, Scientific Journals published an interesting article revealing a structure that has long been missed by traditional anatomy - the interstitium. Because it is widely present throughout the body and involved in many kinds o...

Introduction: Adequate sleep is essential for physical and mental health. Most people need about 8-8.5 hours of sleep every night to feel good. However, scientists have discovered that a genetic mutation can make people sleep only about 5 hours a day...

Common Epitope Tags and SequencesTagProtein SequenceFLAGDYKDDDDK3 x FLAGDYKDHDGDYKDHDIDYKDDDKHAYPYDVPDYAHisHHHHHHHSVQPELAPEDPEDMycEQKLISEEDLV5GKPIPNPLLGLDSTXpressDLDDDDK or DLYDDDDKThrombinLVPRGSBAD (Biotin Acceptor Domain)GLNDIFEAQKIEWHEFactor XaIEG...

Both should work fine, but we always recommend streaking onto agar plates first. If your stocks are contaminated this will often be visible on the plates but very hard to see in broth. Furthermore, if you are counting on doing growth assay and compar...

Introduction Because the human body cannot synthesize or synthesize very little and must be obtained from food, linoleic acid is called an essential fatty acid. And because linoleic acid can lower blood cholesterol and prevent atherosclerosis, it has...

Introduction: For a long time, insulin resistance and elevated blood glucose levels are thought to be the cause of type 2 diabetes. However, in a recently published study, the evidence provided by scientists shows that facts may be very differen...

Sodium azide can interfere during the conjugation reaction. We recommend a maximum concentration of 0.1% sodium azide. Nevertheless, sodium azide is known as an irreversible inhibitor of HRP. We do not recommend performing a HRP conjugation in presen...

For long term storage at 4°C we recommend adding antimicrobial agents and/or stabilizers (e.g. azide, BSA, glycerol, etc.). A new conjugate can be stored for 12-18 months at 4°C as long as the antibody will tolerate storage at 4°C. As the bond betwee...

If you are interested in trying to recover functional protein (as suggested by your interest in Native-PAGE), then you are far better off not using an SDS gel in the first place. Bio-Rad makes an apparatus specifically for running native tube gels an...