-
Product Name
Human UCHL1 (His Tag) recombinant protein
- Documents
-
Description
Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.
-
Protein name
Ubiquitin carboxyl-terminal hydrolase isozyme L1
-
Protein short names
PARK5; PGP 9.5; PGP95; PGP9.5; AW822034; NDGOA; UCHL-1; GAD; UCHL1; R75593; C88048; UCH-L1; HEL-117
-
Uniprot ID
V9HW74
-
Gene Name
UCHL1
-
Source/Expression Host
E. coli
-
Expression Plasmid/cDNA
A DNA sequence encoding the native human UCHL1 (NP_004172.2) (Gln 2-Ala 223) was expressed, with a polyhistide tag at the N-terminus.
-
Protein Species
Human
-
Molecular weight
The recombinant human UCHL1 consisting of 229 amino acids and migrates as an 25.6 kDa band in SDS-PAGE under reducing conditions as predicted.
-
Purity
> 92 % as determined by SDS-PAGE
-
Activity
Measured by the hydrolysis of UbiquitinAMC. The specific activity is >140 pmoles/min/μg.
-
Validations
Human UCHL1 / PGP9.5 Protein (His Tag) SDS-PAGE
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"