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Product Name
UCHL1 antibody
- Documents
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Description
UCHL1 Mouse Monoclonal antibody. Positive IHC detected in human hypothalamus tissue. Positive IF detected in SH-SY5Y cells. Positive WB detected in fetal human brain tissue, mouse brain tissue, pig brain tissue. Observed molecular weight by Western-blot: 27 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human, Mouse, Pig; other species not tested.
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Alternative names
Neuron cytoplasmic protein 9.5 antibody; PARK5 antibody; PGP 9.5 antibody; PGP9.5 antibody; Ubiquitin thioesterase L1 antibody; Uch L1 antibody; UCHL1 antibody
- Immunogen
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Isotype
Mouse IgG1
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Preparation
This antibody was obtained by immunization of UCHL1 recombinant protein (Accession Number: NM_004181). Purification method: Protein G purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:5000-1:50000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
fetal human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:107531(UCHL1 Antibody) at dilution of 1:20000
Immunohistochemistry of paraffin-embedded human hypothalamus tissue slide using Catalog No:107531(UCHL1 Antibody) at dilution of 1:500 (under 10x lens).
Immunohistochemistry of paraffin-embedded human hypothalamus tissue slide using Catalog No:107531(UCHL1 Antibody) at dilution of 1:500 (under 40x lens).
Immunofluorescent analysis of SH-SY5Y cells using Catalog No:107531(UCHL1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)
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Background
UCHL1(Ubiquitin carboxyl-terminal hydrolase isozyme L1) is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. Expression of UCHL1 is highly specific to neurons and to cells of the diffuse neuroendocrine system and their tumors. It is present in all neurons(PMID: 6343558). This protein is present in brain at concentrations at least 50 times greater than in other organs and is a major protein component of neuronal cytoplasm (PMID:7217993). And UCHL1 is a Parkinson's disease susceptibility gene (PMID:15048890).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"