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Product Name
Anti-PGP9.5 (7B8) Mouse antibody
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Description
PGP9.5 (7B8) Mouse monoclonal antibody
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Tested applications
WB, ICC/IF
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Species reactivity
Human, Monkey
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Alternative names
UCHL1 / PGP9.5;UCHL1;B220;CD 45;CD45;cd45 antigen;ec3.1.3.48;GP 180;GP180;Human homolog of severe combined immunodeficiency due to PTPRC deficiency;L CA;L-CA;lca;Leukocyte common antigen;LY 5;LY5;Protein tyrosine phosphatase receptor type C;Protein tyrosi antibody
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Isotype
IgG2b
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Preparation
Antigen: Purified recombinant human UCHL1 / PGP9.5 protein fragments expressed in E.coli.
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Clonality
Monoclonal
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Formulation
Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4 150 mM NaCl) with 0.02% sodium azide 50% glycerol
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/1000
;ICC: 1/300
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Validations
Immunocytochemistry stain of COS7 using UCHL1 / PGP9.5 mouse mAb (1:300).
Western blot detection of UCHL1 / PGP9.5 in U251,IMR32,Raji and COS7 cell lysates and using UCHL1 / PGP9.5 mouse mAb (1:1000 diluted).Predicted band size: 25KDa.Observed band size: 28KDa.
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Background
Swiss-Prot Acc.P09936.PGP9.5 is a single chain transmembraneous glycoprotein which represents the low molecular weight isoform of the Leucocyte Common Antigen (LCA). Hydrolyze isopeptide bonds between the carboxy-terminal glycine of Ub and the ε-amino group of lysine on target proteins and involved in the cotranslational processing of pro-ubiquitin and ribosomal proteins translated as ubiquitin fusions.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"