Anti-UCHL1 antibody

Cat.#: 100685


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Product Information

  • Product Name
    Anti-UCHL1 antibody
  • Documents
  • Description
    Rabbit monoclonal to UCHL1
  • Tested applications
  • Species reactivity
    Rat UCHL1
  • Alternative names
    AW822034 antibody; C88048 antibody; gad antibody; PARK5 antibody; PGP 9.5 antibody; PGP 9.5 antibody; PGP9.5 antibody; PGP9.5 antibody; PGP95 antibody; R75593 antibody; Uchl1 antibody; UCHL1 antibody; UCH-L1 antibody; Uch-L1 antibody; NDGOA antibody; PARK5 antibody; PGP95 antibody; PGP9.5 antibody; Uch-L1 antibody; HEL-117 antibody; gad antibody; C88048 antibody; PGP9.5 antibody; R75593 antibody; UCH-L1 antibody; UCHL-1 antibody; AW822034 antibody
  • Immunogen
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained from a rabbit immunized with purified, recombinant Rat UCHL1 (rR UCHL1; Q00981; Glu2-Ala223).
  • Clonality
  • Formulation
    0.2 μm filtered solution in PBS with 5% trehalose
  • Storage instructions
    This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
    Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
  • Applications

    WB: 2-10 μg/ml

    ELISA: 0.1-0.2 μg/mL

    This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Rat UCHL1. The detection limit for Rat UCHL1 is 0.0078 ng/well.

    IP: 1-4 μg/mg of lysate

  • Validations

    UCHL1 Antibody, Rabbit MAb, Western blot

    UCHL1 Antibody, Rabbit MAb, Western blot

    UCHL1 Antibody, Rabbit MAb, Immunoprecipitation

    UCHL1 Antibody, Rabbit MAb, Immunoprecipitation

  • Background
    Ubiquitin carboxyl-terminal hydrolase isozyme L1, also known as UCH-L1, Ubiquitin thioesterase L1, PGP9.5 and UCHL1, is a deubiqutinating enzyme with important functions in recycling of ubiquitin. Regulated proteolysis by the ubiquitin pathway has been implicated in control of the cell cycle, transcriptional activation, cell fate and growth, and synaptogenesis. The ubiquitin-proteasome system is involved in synaptic plasticity and is proposed to be part of a molecular switch that converts short-term synaptic potentiation to long-term changes in synaptic strength. UCHL1 is found in neuronal cell bodies and processes throughout the neocortex (at protein level). It is expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. UCHL1 is weakly expressed in ovary. UCHL1 is a ubiquitin-protein hydrolase. It is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. UCHL1 also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer of UCHL1 may have ATP-independent ubiquitin ligase activity. UCHL1 dysfunction has been associated with neurodegeneration in Parkinson's, Alzheimer's, and Huntington's disease patients. Reduced UCHL1 function may jeopardize the survival of CNS neurons.
  • References
    • Wada H., et al., 1998, Biochem. Biophys. Res. Commun. 251:688-92.
    • Choi J., et al., 2004, J. Biol. Chem. 279:13256-64.
    • Lombardino,A.2005, et al., J.Proc Natl Acad Sci.USA102 (22):8036-41
    • Okochi-Takada,E. et al., 2006, Int J Cancer. 119 (6):1338-44.
    • Zetterberg, M. et al., 2010, Mol Neurodegener  5 :11.