Rat CD166/ALCAM (Fc Tag) recombinant protein

Rat CD166/ALCAM (Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Activated leukocyte cell adhesion molecule (ALCAM)/Cluster of differentiation (CD166) is a type I transmembrane cell adhesion molecule belonging to the Ig superfamily and a ligand for CD6 that is expressed on T lymphocytes. The extracellular domain of ALCAM contains five Ig-like domains (three Ig-like C2-type domains and two Ig-like V-type domains), of which the amino-terminal V1 domain is essential for ligand binding and ALCAM-mediated cell aggregation. ALCAM mediates both heterophilic (ALCAM-CD6) and homophilic (ALCAM-ALCAM) cell-cell interactions. ALCAM/CD6 interaction plays a role in T cell development and T cell regulation, as well as in the binding of T- and B-cells to activated leukocytes. Recently, homophilic (ALCAM-ALCAM) adhesion was shown to play important roles in tight cell-to-cell interaction and regulation of stem cell differentiation. While expressed in a wide variety of tissues, ALCAM is usually restricted to subsets of cells involved in dynamic growth and/or migration, including neural development, branching organ development, hematopoiesis, immune response and tumor progression. And CD166 is regarded as a potential novel breast cancer indicator and therapeutic target.

CD166 antigen

SC1; ALCAM; CD166; BEN; MEMD; AI853494; MGC27910; DM-GRASP; MUSC

O35112

Alcam

Human Cells

A DNA sequence encoding the rat ALCAM (O35112) (Met1-Lys527) was expressed with the Fc region of human IgG1 at the C-terminus.

Rat

The recombinant rat ALCAM/Fc is a disulfide-linked homodimer. The reduced monomer comprises 741 amino acids and has a predicted molecular mass of 83.1 kDa. The apparent molecular mass of the protein is approximately 94 kDa in SDS-PAGE under reducing conditions due to glycosylation.

> 95 % as determined by SDS-PAGE

Immobilized rat CD6-His (Cat:500412) at 10 μg/ml (100 μl/well) can bind rat ALCAM-Fc3, The EC₅₀ of rat ALCAM-Fc3 is 19.5-45.5 ng/ml.