Mouse CD166/ALCAM (His Tag) recombinant protein

Mouse CD166/ALCAM (His Tag) recombinant protein

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Activated leukocyte cell adhesion molecule (ALCAM)/Cluster of differentiation (CD166) is a type I transmembrane cell adhesion molecule belonging to the Ig superfamily and a ligand for CD6 that is expressed on T lymphocytes. The extracellular domain of ALCAM contains five Ig-like domains (three Ig-like C2-type domains and two Ig-like V-type domains), of which the amino-terminal V1 domain is essential for ligand binding and ALCAM-mediated cell aggregation. ALCAM mediates both heterophilic (ALCAM-CD6) and homophilic (ALCAM-ALCAM) cell-cell interactions. ALCAM/CD6 interaction plays a role in T cell development and T cell regulation, as well as in the binding of T- and B-cells to activated leukocytes. Recently, homophilic (ALCAM-ALCAM) adhesion was shown to play important roles in tight cell-to-cell interaction and regulation of stem cell differentiation. While expressed in a wide variety of tissues, ALCAM is usually restricted to subsets of cells involved in dynamic growth and/or migration, including neural development, branching organ development, hematopoiesis, immune response and tumor progression. And CD166 is regarded as a potential novel breast cancer indicator and therapeutic target.

Putative uncharacterized protein

MEMD; MUSC; DM-GRASP; MGC27910; AI853494; ALCAM; SC1; BEN; CD166

Q61490

Alcam

Human Cells

A DNA sequence encoding the extracellular domain (Met 1-Lys 527) of mouse ALCAM (NP_033785.1) precursor was expressed, fused with a polyhistidine tag at the C-terminus.

Mouse

The recombinant mouse ALCAM consists of 511 amino acids and has a predicted molecular mass of 57.7 kDa. Due to glycosylation, rmALCAM migrates as an approximately 70-80 kDa band in SDS-PAGE under reducing conditions.

> 98 % as determined by SDS-PAGE

Immobilized mouse ALCAM-His at 10 μg/ml (100 μl/well) can bind mouse CD6-Fc (Cat:500293), The EC₅₀ of mouse CD6-Fc (Cat:500293) is 0.08-0.18 μg/ml.