Mouse CD166/ALCAM (His & Fc Tag) recombinant protein

Mouse CD166/ALCAM (His & Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Activated leukocyte cell adhesion molecule (ALCAM)/Cluster of differentiation (CD166) is a type I transmembrane cell adhesion molecule belonging to the Ig superfamily and a ligand for CD6 that is expressed on T lymphocytes. The extracellular domain of ALCAM contains five Ig-like domains (three Ig-like C2-type domains and two Ig-like V-type domains), of which the amino-terminal V1 domain is essential for ligand binding and ALCAM-mediated cell aggregation. ALCAM mediates both heterophilic (ALCAM-CD6) and homophilic (ALCAM-ALCAM) cell-cell interactions. ALCAM/CD6 interaction plays a role in T cell development and T cell regulation, as well as in the binding of T- and B-cells to activated leukocytes. Recently, homophilic (ALCAM-ALCAM) adhesion was shown to play important roles in tight cell-to-cell interaction and regulation of stem cell differentiation. While expressed in a wide variety of tissues, ALCAM is usually restricted to subsets of cells involved in dynamic growth and/or migration, including neural development, branching organ development, hematopoiesis, immune response and tumor progression. And CD166 is regarded as a potential novel breast cancer indicator and therapeutic target.

Putative uncharacterized protein

DM-GRASP; MUSC; AI853494; MGC27910; MEMD; BEN; CD166; ALCAM; SC1

Q61490

Alcam

Human Cells

A DNA sequence encoding the extracellular domain (Met 1-Lys 527) of mouse ALCAM (NP_033785.1) precursor was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.

Mouse

The recombinant mouse ALCAM/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer consists of 748 amino acids and has a predicted molecular mass of 84.2 kDa. Due to glycosylation, in SDS-PAGE under reducing conditions, rmALCAM/Fc migrates with approximately molecular mass of 105-110 kDa & 210 kDa corresponding to the monomer and homodimer.

> 95 % as determined by SDS-PAGE

Immobilized mouse CD6-His (Cat:503645) at 10 μg/ml (100 μl/well) can bind mouse ALCAM-Fc2h, The EC₅₀ of mouse ALCAM-Fc2h is 0.12-0.28 μg/ml.