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Product Name
Mouse FGFR3/CD333 (His & Fc Tag) recombinant protein
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Description
FGFR3, also known as CD333, is a member of the fibroblast growth factor receptor (FGFR) family, with its amino acid sequence being highly conserved between members and among divergent species. FGFR family members differ from one another in their ligand affinities and tissue distribution. FGFRs are transmembrane catalytic receptors that have intracellular tyrosine kinase activity. Mutations in FGFR genes are the cause of several human developmental disorders characterized by skeletal abnormalities such as achondroplasia, and upregulation of FGFR expression may lead to cell transformation and cancer. FGFR3, a full-length representative protein would consist of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of FGFR3 interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. FGFR3 binds acidic and basic fibroblast growth hormone and plays a role in bone development and maintenance. Mutations in FGFR3 gene lead to craniosynostosis and multiple types of skeletal dysplasia. Three alternatively spliced transcript variants that encode different protein isoforms have been described. CD333 is the receptor for acidic and basic fibroblast growth factors.
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Protein name
Fibroblast growth factor receptor
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Protein short names
FGFR-3; CD333; CEK2; HBGFR; HSFGFR3EX; ACH; MFR3; FR3; JTK4; FGFR3; SAM3; FLG-2
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Uniprot ID
Q7TSI8
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Gene Name
Fgfr3; mCG_16331
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Source/Expression Host
Human Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the extracellular domain (Met 1-Tyr 367) of mouse FGFR3 (NP_032036.2) precursor was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
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Protein Species
Mouse
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Molecular weight
The recombinant mouse FGFR3/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer consists of 595 amino acids and has a predicted molecular mass of 66 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm FGFR3/Fc monomer is approximately 100-110 kDa due to glycosylation.
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Purity
> 92 % as determined by SDS-PAGE
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Activity
Measured by its ability to bind mouse aFGF in a functional ELISA.
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Validations
Mouse FGFR3 / CD333 Protein (His & Fc Tag) SDS-PAGE
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