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Product Name
Anti-FGFR3/CD333 antibody
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Description
Rabbit polyclonal to FGFR3/CD333
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Tested applications
ELISA, WB, IHC-P
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Species reactivity
Cynomolgus FGFR3 / CD333
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Alternative names
ACH antibody; CEK2 antibody; JTK4 antibody; CD333 antibody; HSFGFR3EX antibody; FR3 antibody; Mfr3 antibody; sam3 antibody; CD333 antibody; Flg-2 antibody; HBGFR antibody; Fgfr-3 antibody; ACH antibody; CD333 antibody; CEK2 antibody; FGFR3 antibody; Fgfr3 antibody; Fgfr-3 antibody; HBGFR antibody; HSFGFR3EX antibody; JTK4 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Cynomolgus FGFR3 / CD333 (XP_005554344.1; Met1-Gly375). FGFR3 / CD333 specific IgG was purified by Cynomolgus FGFR3 / CD333 affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS with 5% trehalose
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 10-20 μg/ml
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Cynomolgus FGFR3 / CD333. The detection limit for Cynomolgus FGFR3 / CD333 is < 0.039 ng/well.
IHC-P: 0.1-2 μg/mL
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Validations
FGFR3 / CD333 Antibody, Rabbit PAb, Antigen Affinity Purified
Immunochemical staining of cynomolgus FGFR3 in cynomolgus brain with rabbit polyclonal antibody (0.5 µg/mL, formalin-fixed paraffin embedded sections).
FGFR3 / CD333 Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
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Background
FGFR3, also known as CD333, is a member of the fibroblast growth factor receptor (FGFR) family, with its amino acid sequence being highly conserved between members and among divergent species. FGFR family members differ from one another in their ligand affinities and tissue distribution. FGFRs are transmembrane catalytic receptors that have intracellular tyrosine kinase activity. Mutations in FGFR genes are the cause of several human developmental disorders characterized by skeletal abnormalities such as achondroplasia, and upregulation of FGFR expression may lead to cell transformation and cancer. FGFR3, a full-length representative protein would consist of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of FGFR3 interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. FGFR3 binds acidic and basic fibroblast growth hormone and plays a role in bone development and maintenance. Mutations in FGFR3 gene lead to craniosynostosis and multiple types of skeletal dysplasia. Three alternatively spliced transcript variants that encode different protein isoforms have been described. CD333 is the receptor for acidic and basic fibroblast growth factors.
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References
- Keegan K, et al. (1991) Isolation of an additional member of the fibroblast growth factor receptor family, FGFR-3. Proc Natl Acad Sci. 88(4):1095-9.
- Hafner C, et al. (2007) FGFR3 mutations in epidermal nevi and seborrheic keratoses: lessons from urothelium and skin. J Invest Dermatol. 127(7):1572-3.
- Lamy A, et al. (2006) Molecular profiling of bladder tumors based on the detection of FGFR3 and TP53 mutations. J Urol. 176(6 Pt 1):2686-9.
- Schweitzer DN, et al. (2001) Subtle radiographic findings of achondroplasia in patients with Crouzon syndrome with acanthosis nigricans due to an Ala391Glu substitution in FGFR3. Am J Med Genet. 98 (1):75-91.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"