Mouse FGFR3/CD333 (His Tag) recombinant protein

Mouse FGFR3/CD333 (His Tag) recombinant protein

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FGFR3, also known as CD333, is a member of the fibroblast growth factor receptor (FGFR) family, with its amino acid sequence being highly conserved between members and among divergent species. FGFR family members differ from one another in their ligand affinities and tissue distribution. FGFRs are transmembrane catalytic receptors that have intracellular tyrosine kinase activity. Mutations in FGFR genes are the cause of several human developmental disorders characterized by skeletal abnormalities such as achondroplasia, and upregulation of FGFR expression may lead to cell transformation and cancer. FGFR3, a full-length representative protein would consist of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of FGFR3 interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. FGFR3 binds acidic and basic fibroblast growth hormone and plays a role in bone development and maintenance. Mutations in FGFR3 gene lead to craniosynostosis and multiple types of skeletal dysplasia. Three alternatively spliced transcript variants that encode different protein isoforms have been described. CD333 is the receptor for acidic and basic fibroblast growth factors.

Fibroblast growth factor receptor

FR3; MFR3; ACH; HSFGFR3EX; FGFR-3; HBGFR; CEK2; CD333; FLG-2; FGFR3; SAM3; JTK4

Q7TSI8

Fgfr3; mCG_16331

Human Cells

A DNA sequence encoding the extracellular domain (Met 1-Tyr 367) of mouse FGFR3 (NP_032036.2) was expressed, fused with a polyhistidine tag at the C-terminus.

Mouse

The recombinant mouse FGFR3 comprises 358 amino acids and has a calculated molecular mass of 37 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmFGFR3 is approximately 70-80 kDa due to glycosylation.

> 98 % as determined by SDS-PAGE

Measured by its ability to inhibit FGF acidic dependent proliferation of Balb/c3T3 mouse embryonic fibroblasts.
The ED₅₀ for this effect is typically 0.3-4 μg/mL.