Human EGFR/HER1/ErbB1 (His Tag) recombinant protein

Human EGFR/HER1/ErbB1 (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.

Epidermal growth factor receptor

RP23-295E4.1; WA-2; AI552599; EGFR; ERBB1; ERRP; WA2; NISBD2; ERBB; ERRB1; ERBB-1; 9030024J15RIK; WA5; PIG61; MENA; HER1

P00533

EGFR; ERBB; ERBB1; HER1

Baculovirus-Insect Cells

A DNA sequence encoding the extracellular domain (Met 1-Lys 642) of human EGFR (NP_005219) was expressed with a C-terminal polyhistidine tag.

Human

The recombinant human EGFR consists of 626 amino acids and has a calculated molecular mass of 69.2 kDa. The recombinant protein migrates as an approximately 75 kDa protein in SDS-PAGE under reducing conditions.

> 95 % as determined by SDS-PAGE

Measured by its binding ability in a functional ELISA.
Immobilized human EGF at 10 μg/ml can bind human EGFR with a linear range of 3.2-400 ng/ml.