Anti-EGFR (5E10) Mouse antibody

Cat.#: 168093

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Product Information

  • Product Name
    Anti-EGFR (5E10) Mouse antibody
  • Documents
  • Description
    EGFR (5E10) Mouse monoclonal antibody
  • Tested applications
    WB, ICC/IF, IP
  • Species reactivity
    Human, Monkey
  • Alternative names
    Avian erythroblastic leukemia viral (v erb b) oncogene homolog;Cell growth inhibiting protein 40;Cell proliferation inducing protein 61;EGF R;EGFR;EGFR_HUMAN;Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) antibody
  • Isotype
    Mouse IgG1
  • Preparation
    Antigen: Purified recombinant human EGFR protein fragments expressed in E.coli.
  • Clonality
    Monoclonal
  • Formulation
    Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
  • Storage instructions
    Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Applications

    WB: 1/1000

    ;ICC: 1/200

  • Validations

    Immunocytochemistry staining of HeLa cells using EGFR mouse mAb (dilution 1:200).

    Immunocytochemistry staining of HeLa cells using EGFR mouse mAb (dilution 1:200).

    Immunoprecipitation analysis of Hela cell lysates using EGFR mouse mAb.

    Immunoprecipitation analysis of Hela cell lysates using EGFR mouse mAb.

    Western blot detection of EGFR in A549,MDA-MB-468 and COS7 cell lysates using EGFR mouse mAb(dilution 1:1000).Predicted band size:134 Kda.Observed band size:175KDa.

    Western blot detection of EGFR in A549,MDA-MB-468 and COS7 cell lysates using EGFR mouse mAb(dilution 1:1000).Predicted band size:134 Kda.Observed band size:175KDa.

    Immunocytochemistry staining of MDA-MB-468 cells fixed with 4% Paraformaldehyde and using EGFR mouse mAb (dilution 1:200).

    Immunocytochemistry staining of MDA-MB-468 cells fixed with 4% Paraformaldehyde and using EGFR mouse mAb (dilution 1:200).

  • Background
    Swiss-Prot Acc.P00533.EGFR is a receptor tyrosine kinase. Receptor for epidermal growth factor (EGF) and related growth factors including TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentiation. . A single-pass transmembrane tyrosine kinase. Ligand binding to this receptor results in receptor dimerization, autophosphorylation (in trans), activation of various downstream signaling molecules and lysosomal degradation.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"