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Product Name
Anti-EGFR (5E10) Mouse antibody
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Description
EGFR (5E10) Mouse monoclonal antibody
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Tested applications
WB, ICC/IF, IP
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Species reactivity
Human, Monkey
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Alternative names
Avian erythroblastic leukemia viral (v erb b) oncogene homolog;Cell growth inhibiting protein 40;Cell proliferation inducing protein 61;EGF R;EGFR;EGFR_HUMAN;Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) antibody
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Isotype
IgG1
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Preparation
Antigen: Purified recombinant human EGFR protein fragments expressed in E.coli.
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Clonality
Monoclonal
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Formulation
Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/1000
;ICC: 1/200
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Validations
Immunocytochemistry staining of HeLa cells using EGFR mouse mAb (dilution 1:200).
Immunoprecipitation analysis of Hela cell lysates using EGFR mouse mAb.
Western blot detection of EGFR in A549,MDA-MB-468 and COS7 cell lysates using EGFR mouse mAb(dilution 1:1000).Predicted band size:134 Kda.Observed band size:175KDa.
Immunocytochemistry staining of MDA-MB-468 cells fixed with 4% Paraformaldehyde and using EGFR mouse mAb (dilution 1:200).
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Background
Swiss-Prot Acc.P00533.EGFR is a receptor tyrosine kinase. Receptor for epidermal growth factor (EGF) and related growth factors including TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentiation. . A single-pass transmembrane tyrosine kinase. Ligand binding to this receptor results in receptor dimerization, autophosphorylation (in trans), activation of various downstream signaling molecules and lysosomal degradation.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"