Cart 2
  • Anti-EGFR Rabbit antibody
  • Anti-EGFR Rabbit antibody

Anti-EGFR Rabbit antibody

Cat.#: 167184

Size:

Special Price 441.3 USD

Availability: In Stock
- +

Add to cart to get an online quotation

Product Information

  • Product Name
    Anti-EGFR Rabbit antibody
  • Documents
  • Description
    EGFR Rabbit polyclonal antibody
  • Tested applications
    WB, ICC/IF, FC
  • Species reactivity
    Human, Mouse
  • Alternative names
    ERBB; HER1; mENA; ERBB1; PIG61; NISBD2 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: A synthetic peptide of human EGFR
  • Clonality
    Polyclonal antibody
  • Formulation
    Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
  • Storage instructions
    Store at -20°C. Stable for 12 months from date of receipt.
  • Applications

    WB: 1/1000

    ICC/IF: 1/50

    FC: 1/20

  • Validations

    Immunocytochemistry of EGFR(green) in Hela cells using EGFR Rabbit pAb at dilution 1/50, and DAPI(blue)

    Immunocytochemistry of EGFR(green) in Hela cells using EGFR Rabbit pAb at dilution 1/50, and DAPI(blue)

    Western blot detection of EGFR in Hela cell lysates using EGFR Rabbit pAb(1:1000 diluted).Predicted band size:134kDa.Observed band size:175kDa.

    Western blot detection of EGFR in Hela cell lysates using EGFR Rabbit pAb(1:1000 diluted).Predicted band size:134kDa.Observed band size:175kDa.

  • Background
    Swiss-Prot Acc.P00533.Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses (PubMed:2790960, PubMed:10805725, PubMed:27153536). Known ligands include EGF, TGFA/TGF-alpha, AREG, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF (PubMed:2790960, PubMed:7679104, PubMed:8144591, PubMed:9419975, PubMed:15611079, PubMed:12297049, PubMed:27153536, PubMed:20837704). Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules (PubMed:27153536). May also activate the NF-kappa-B signaling cascade (PubMed:11116146). Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling (PubMed:11602604). Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin (PubMed:11483589). Plays a role in enhancing learning and memory performance .
  • References

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"