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Product Name
Anti-Cleaved-Caspase-3 p17 Rabbit antibody
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Description
Cleaved-Caspase-3 p17 Rabbit polyclonal antibody
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Tested applications
WB, IHC-P, ICC/IF
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Species reactivity
Human
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthesized peptide derived from human active Caspase-3
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Clonality
Polyclonal
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Formulation
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage instructions
Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
1:500~1:2000
1:50~1:100
1:50~1:100
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Validations
Western blot analysis of active Caspase-3 expression in Jurkat cell lysate treated with Camptothecin.
Immunofluorescent analysis of HepG2 cells, using active Caspase-3 Antibody .
Western blot analysis of active Caspase-3 expression in Jurkat,C6 and Hela cell lysates using active Caspase-3 antibody at 1/1000 dilution.Predicted band size:17KDa.Observed band size:35,32,17KDa.
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Background
Swiss-Prot Acc.P42574.Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme.
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References
- Iron overload resulting from the chronic oral administration of ferric citrate induces parkinsonism phenotypes in middle-aged mice
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"