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Product Name
Anti-Caspase-3 Rabbit antibody
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Description
Caspase-3 Rabbit polyclonal antibody
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Tested applications
WB
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Species reactivity
Human, Mouse
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Alternative names
Caspase-3 antibody; CASP-3 antibody; Apopain antibody; Cysteine protease CPP32 antibody; CPP-32 antibody; Protein Yama antibody; SREBP cleavage activity 1 antibody; SCA-1 antibody; Caspase-3 subunit p17 antibody; Caspase-3 subunit p12 antibody; CASP3 antibody; CPP32. antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Purified recombinant human Caspase-3 protein fragments expressed in E.coli.
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Clonality
Polyclonal
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Formulation
PBS(pH 7.4) containing with 0.03% Proclin300 and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/1000
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Validations
Western blot detection of Caspase-3 in Jurkat and 3T3 cell lysates using Caspase-3 Rabbit pAb (1:1000 diluted). Predicted band size: 35KDa. Observed band size:35KDa.
Western blot analysis of extracts from Jurkat cells, untreated (-) or treated with Staurosporine (1 μM; 3 hr), using Caspase-3 Rabbit pAb (166660, upper) or β-Actin (8F10) Mouse mAb (lower).
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Background
Swiss-Prot Acc.P42574.Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"