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Product Name
Anti-Caspase-3 Rabbit antibody
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Description
Caspase-3 Rabbit polyclonal antibody
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Tested applications
WB, IHC-P, IP
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Species reactivity
Human, Mouse, Rat
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Alternative names
Caspase-3 antibody; CASP-3 antibody; Apopain antibody; Cysteine protease CPP32 antibody; CPP-32 antibody; Protein Yama antibody; SREBP cleavage activity 1 antibody; SCA-1 antibody; Caspase-3 subunit p17 antibody; Caspase-3 subunit p12 antibody; CASP3 antibody; CPP32. antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant protein of human Caspase-3
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Clonality
Polyclonal
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Formulation
Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Storage instructions
Store at -20°C. Stable for 12 months from date of receipt.
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Applications
WB: 1/1000
IHC: 1/50
IP: 1/20
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Validations
Western blot detection of Caspase-3 in K562,Rat Brain,C6,3T3,Hela cell lysates using Caspase-3 Rabbit pAb(1:1000 diluted).Predicted band size:32kDa.Observed band size:32kDa.
Immunohistochemistry of Caspase-3 in paraffin-embedded Human tonsil using Caspase-3 Rabbit pAb at dilution 1/100
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Background
Swiss-Prot Acc.P42574.Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"