Amino Acid Stock Solutions

Amino acid

Stock Concentration

Molecular Weight

gm/100ml

ml/liter

ml/plate

Arginine

50mM

100mM

210.7

1.054

2.107

10

5

0.2

0.1

Asparagine

300 mM

132.1

7.926

10

0.2

Histidine HCl

100 mM

209

2.09

3

0.1

Isoleucine

50 mM

131.2

0.656

10

0.2

Leucine

100 mM

131

1.31

20

0.2

Lysine

100 mM

182.7

1.827

10

0.1

Methionine

50 mM

149.2

.0746

20

0.15

Phenylalanine

30 mM

165.2

.0.5

10

0.2

Proline

100 mM

115.1

1.15

10

0.2

Serine

100 mM

105.1

1.05

10

0.2

Threonine

300 mM

119.1

3.57

5

0.1

Tryptophan1

40 mM

204.2

0.8

10

0.2

Tyrosine2

7 mM

181.2

0.127

10

0.2

Valine

50 mM

117.5

0.59

3.4

0.07

Add few drops of NaOH to solubilize as necessary.

1. Filter sterilize, do not autoclave and do not make more than 100ml as deteriorates in 2-3 months.

2. Filter sterilize, do not autoclave.

Souce: NovoPro    2020-10-07