Green Indicator Plates

Jeff Lawrence (Z51)

(NOTE: This section describes classic green plates, which have now been superceded by "Mint Green Plates")

Plate Mix

	828 g	Bacto-Tryptone (Difco)
	105 g	Yeast Extract (Difco)
	516 g	NaCl
	1551 g	Agar
	6.8 g	Aniline Blue (C32H27N3O9S3·2Na)
 	64.4 g	Alizarin Yellow G (C13H8N3NaO5)

1 Place ingredients into a plastic tub and shake vigorously to distribute dyes well.

Normal recipe - 4 L of green plates:

2. Autoclave 116 g Plate Mix in 4L water.

3. When cool enough to pour, add 67.2 mL of 50% Glucose.

Small Recipe:

Make Dye Stock as:

	6.5 mg/ml	Aniline Blue	1.3 g	Aniline Blue
	62.2 mg/ml	Alizarin Yellow	12.4 g	Alizarin Yellow
					200 mL	Water

Combine and Autoclave:

	8 g	Bacto-Tryptone (Difco)
	1 g	Yeast Extract (Difco)
	5 g	NaCl
	15 g	Agar
	10 mL	Dye Stock
	990 mL	Water

After cooling, add 16.8 mL of 50% Glucose and pour.

Souce: NovoPro    2020-10-07