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Product Name
Canine VEGF / VEGFA / VEGF165 recombinant protein
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Description
Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin (By similarity).
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Protein name
Vascular endothelial growth factor A
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Protein short names
MGC70609; MVCD1; VPF; VEGF; VEGF-A; RP1-261G23.1
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Uniprot ID
Q9MYV3
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Gene Name
VEGFA; VEGF
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Source/Expression Host
Human Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the canine VEGFA(NP_001103972.1) (Met1-Arg190) was expressed.
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Protein Species
Canine
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Molecular weight
The recombinant canine VEGFA comprises 164 amino acids and has a predicted molecular mass of 19.1 kDa. The apparent molecular mass of the protein is approximately 23 kDa in SDS-PAGE under reducing conditions due to glycosylation.
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Purity
> 95 % as determined by SDS-PAGE
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Activity
Immobilized canine VEGFA at 10 μg/ml (100 μl/well) can bind human VEGFR2-Fc (Cat:501107), The EC50 of human VEGFR2-Fc (Cat:501107) is 33.83-78.95 ng/ml.
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Validations
Canine VEGF / VEGFA Protein SDS-PAGE
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