Human VEGF / VEGFA / VEGF165 recombinant protein

Human VEGF / VEGFA / VEGF165 recombinant protein

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Vascular endothelial growth factor (VEGF), also known as vascular permeability factor (VPF) and VEGF-A, is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is a member of the platelet-derived growth factor (PDGF)/vascular endothelial growth factor (VEGF) family and often exists as a disulfide-linked homodimer. VEGF-A protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, inhibiting apoptosis and tumor growth. VEGF-A protein is also a vasodilator that increases microvascular permeability, thus it was originally referred to as vascular permeability factor.

Vascular endothelial growth factor A

RP1-261G23.1; VEGF; VPF; VEGF-A; MVCD1; MGC70609

P15692

VEGFA; VEGF

Baculovirus-Insect Cells

A DNA sequence encoding the human VEGF 165 isoform (P15692-4) (Met1-Arg191) was expressed. Human and Cynomolgus VEGF165 sequences are identical.

Human

The recombinant human VEGF consists of 165 amino acids after removal of the signal peptide and predicts a molecular mass of 19.2 kDa. Due to different glycosylation, the apparent molecular mass of rhVEGF165 is approximately 20 and 22 kDa in SDS-PAGE under reducing conditions, corresponding to the monomer.

> 98 % as determined by SDS-PAGE

Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC).
The ED₅₀ for this effect is typically 3-12 ng/ml.