-
Product Name
Human TNFR2/CD120b/TNFRSF1B (aa 1-268, 196 Met/Arg, His Tag) recombinant protein
- Documents
-
Description
Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.
-
Protein name
Tumor necrosis factor receptor superfamily member 1B
-
Protein short names
TNFR-II; TBPII; TNF-R75; P75TNFR; TNF-R-II; TNFRII; P75 TNF RECEPTOR; TNFR-1; TNFRSF1B; TNFR2; P75; CD120B; P75 TNFR; TNF-R2; TNF-ALPHAR2; TNFR1B; TNFALPHA-R2; RP23-157J11.1; TNFR80; TNF-RII; TNFBR
-
Uniprot ID
P20333
-
Gene Name
TNFRSF1B; TNFBR; TNFR2
-
Source/Expression Host
Human Cells
-
Expression Plasmid/cDNA
A DNA sequence encoding the human TNFRSF1B (P20333-2) (Met1-Arg268, natural variant 196 Met/Arg) was expressed with a C-terminal polyhistidine tag.
-
Protein Species
Human
-
Molecular weight
The recombinant human TNFRSF1B comprises 257 amino acids and has a predicted molecular mass of 27.7 kDa. The apparent molecular mass of the protein is approximately 43 kDa in SDS-PAGE under reducing conditions due to glycosylation.
-
Purity
> 95 % as determined by SDS-PAGE
-
Activity
Measured by its ability to inhibit the TNF-α mediated cytotoxicity in the L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D.
The ED50 for this effect is typically 5-40 ng/mL in the presence of 1 ng/mL recombinant human TNF-α. -
Validations
Human TNFR2 / CD120b / TNFRSF1B Protein (aa 1-268, 196 Met/Arg, His Tag) SDS-PAGE
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"