Human TNFR2/CD120b/TNFRSF1B (His Tag) recombinant protein

Human TNFR2/CD120b/TNFRSF1B (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Tumor necrosis factor receptor superfamily, member 1B (TNFRSF1B), also known as Tumor necrosis factor receptor 2 (TNFR2) or CD120b antigen, is a member of the tumor necrosis factor receptor superfamily. TNFR2/CD120b/TNFRSF1B is a member of the TNF-receptor superfamily. This protein and TNF-receptor 1 form a heterocomplex that mediates the recruitment of two anti-apoptotic proteins, c-IAP1 and c-IAP2, which possess E3 ubiquitin ligase activity. Knockout studies in mice also suggest a role of this protein in protecting neurons from apoptosis by stimulating antioxidative pathways. TNFR2/CD120b/TNFRSF1B is not a major contributing factor to the genetic risk of type 2 diabetes, its associated peripheral neuropathy and hypertension and related metabolic traits in North Indians. Tumor necrosis factor receptor superfamily, member 1B (TNFRSF1B) has been reported to be associated with SLE risk in Japanese populations. TNFR2/CD120b/TNFRSF1B serves as a receptor with high affinity for TNFSF2 and approximately 5-fold lower affinity for homotrimeric TNFSF1. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.

Tumor necrosis factor receptor superfamily member 1B

TNFR-II; TBPII; TNF-R75; P75TNFR; TNFRII; TNF-R-II; P75 TNF RECEPTOR; TNFR-1; TNFRSF1B; TNFR2; P75; CD120B; P75 TNFR; TNF-R2; TNFR1B; TNF-ALPHAR2; RP23-157J11.1; TNFALPHA-R2; TNFR80; TNF-RII; TNFBR

P20333

TNFRSF1B; TNFBR; TNFR2

Human Cells

A DNA sequence encoding the extracellular domain (Met 1-Asp 257) of human TNFRII (NP_001057.1) was expressed, fused with a C-terminal polyhistidine tag.

Human

The recombinant human TNFRII comprises 246 amino acids and has a calculated molecular mass of 26.7 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh TNFRII is approximately 42-45 kDa due to glycosylation.

> 97 % as determined by SDS-PAGE

Measured by its ability to inhibit the TNF-α mediated cytotoxicity in the L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED₅₀ for this effect is typically 0.2-0.8 μg/ml in the presence of 0.25 ng/mL recombinant human TNF-α.