Human TNFR2/CD120b/TNFRSF1B (His & Fc Tag) recombinant protein

Human TNFR2/CD120b/TNFRSF1B (His & Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Tumor necrosis factor receptor superfamily, member 1B (TNFRSF1B), also known as Tumor necrosis factor receptor 2 (TNFR2) or CD120b antigen, is a member of the tumor necrosis factor receptor superfamily. TNFR2/CD120b/TNFRSF1B is a member of the TNF-receptor superfamily. This protein and TNF-receptor 1 form a heterocomplex that mediates the recruitment of two anti-apoptotic proteins, c-IAP1 and c-IAP2, which possess E3 ubiquitin ligase activity. Knockout studies in mice also suggest a role of this protein in protecting neurons from apoptosis by stimulating antioxidative pathways. TNFR2/CD120b/TNFRSF1B is not a major contributing factor to the genetic risk of type 2 diabetes, its associated peripheral neuropathy and hypertension and related metabolic traits in North Indians. Tumor necrosis factor receptor superfamily, member 1B (TNFRSF1B) has been reported to be associated with SLE risk in Japanese populations. TNFR2/CD120b/TNFRSF1B serves as a receptor with high affinity for TNFSF2 and approximately 5-fold lower affinity for homotrimeric TNFSF1. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.

Tumor necrosis factor receptor superfamily member 1B

RP23-157J11.1; TNFALPHA-R2; TNF-ALPHAR2; TNFR1B; TNF-R2; P75 TNFR; CD120B; TNFBR; TNF-RII; TNFR80; P75TNFR; TNF-R75; TBPII; TNFR-II; TNFR2; P75; TNFRSF1B; TNFR-1; P75 TNF RECEPTOR; TNFRII; TNF-R-II

P20333

TNFRSF1B; TNFBR; TNFR2

Human Cells

A DNA sequence encoding the extracellular domain (Met 1-Asp 257) of human TNFRII (NP_001057.1) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.

Human

The recombinant human TNFRII/Fc is a disulfide-linked homodimer. The reduced monomer consists of 482 amino acids and has a predicted molecular mass of 53 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh TNFRII/Fc monomer is approximately 75 kDa due to glycosylation.

> 95 % as determined by SDS-PAGE

Measured by its ability to inhibit the TNF-α mediated cytotoxicity in the L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED₅₀ for this effect is typically 4-16 ng/ml in the presence of 0.25 ng/mL recombinant human TNF-α.