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Product Name
Anti-TNFR2/CD120b/TNFRSF1B antibody
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Description
Rabbit monoclonal to TNFR2/CD120b/TNFRSF1B
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Tested applications
B/N
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Species reactivity
Human TNFRSF1B / TNFR2 / CD120b
No cross-reactivity with Mouse TNFR2 (504311) in ELISA assay -
Alternative names
Tnfbr antibody; p75 antibody; CD120b antibody; CD120b antibody; p75 antibody; p75 TNF receptor antibody; p75 TNFR antibody; p75TNFR antibody; RP23-157J11.1 antibody; TBPII antibody; TNF-alphaR2 antibody; TNFalpha-R2 antibody; TNFBR antibody; Tnfr-1 antibody; TNFR1B antibody; TNFR2 antibody; Tnfr2 antibody; TNF-R2 antibody; TNF-R2 antibody; TNF-R75 antibody; TNF-R75 antibody; TNFR80 antibody; TNFR80 antibody; TNFRII antibody; TNF-RII antibody; TNF-RII antibody; TNFR-II antibody; TNFR-II antibody; TNF-R-II antibody; TNF-R-II antibody; TNFRSF1B antibody; Tnfrsf1b antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human TNFRSF1B / CD120b (rh TNFRSF1B; Met 1-Asp 257; NP_001057.1).
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Clonality
Monoclonal
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Formulation
0.2 μm filtered solution in Histidine and Arginine buffer containing 120mM NaCl, 0.02% Tween 80, pH6.0
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
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Applications
Block - This TNFR2 neutralizing antibody can block the binding of recombinant TNFa to its recombinant receptor TNFRII.
Neutralization - The neutralization activity of TNFR2/TNFRSF1B Neutralizing Antibody is Measured by its ability to neutralize TNFR2/TNFRSF1B-mediated inhibition of cytotoxicity in the L‑929 mouse fibroblast cell line. The Neutralization titer (IC50) is typically 1-3 µg/mL in the presence of 1 μg/mL Recombinant Human TNFR2/TNFRSF1B, 0.25 ng/mL Recombinant Human TNFa, and 1 µg/mL actinomycin D. -
Validations
TNFR2 / CD120b / TNFRSF1B Neutralizing Antibody
TNFR2/TNFRSF1B-mediated inhibition of cytotoxicity was Neutralized by Human TNFR2 Antibody. Recombinant Human TNFR2/TNFRSF1B (Catalog 10417-H08H) inhibits Recombinant Human TNFa (Catalog 10602-HNAE) induced cytotoxicity in the L-929 mouse fibroblast cell line. Inhibition of Recombinant Human TNFa (0.25 ng/mL) activity elicited by Recombinant Human TNFR2/TNFRSF1B (1 µg/mL) is neutralized by increasing concentrations of Human TNFR2/TNFRSF1B Monoclonal Antibody (Catalog 10417-R00N6). The IC50 is typically 1-3 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).
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Background
Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"