Rat IL12B/IL-12B (His Tag) recombinant protein

Rat IL12B/IL-12B (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Subunit beta of interleukin 12 (also known as natural killer cell stimulatory factor 2, or cytotoxic lymphocyte maturation factor 2, p40) (IL12B) is a subunit of human interleukin 12. IL12B/IL-12B is a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. Interleukin 12 is a disulfide-linked heterodimer composed of the 40 kD cytokine receptor like subunit encoded by this gene, and a 35 kD subunit encoded by IL12A. IL12B/IL-12B is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. This cytokine has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Overexpression of this gene was observed in the central nervous system of patients with multiple sclerosis (MS), suggesting a role of this cytokine in the pathogenesis of the disease. The promoter polymorphism of this gene has been reported to be associated with the severity of atopic and non-atopic asthma in children. IL12B/IL-12B associates with IL23A to form the IL-23 interleukin, an heterodimeric cytokine which functions in innate and adaptive immunity.

Interleukin-12 subunit beta

IMD28; IL12B; NKSF; CLMF2; CLMF; P40; IL12P40; RP23-388G23.1; IMD29; IL-12B; NKSF2; IL-12P40

E9PU71

Il12b; IL12B; rCG_35081

Human Cells

A DNA sequence encoding the rat IL12B (G3V9Y5) (Met1-Ser335) was expressed with a polyhistidine tag at the C-terminus.

Rat

The recombinant rat IL12B comprises 324 amino acids and predicts a molecular mass of 37.2 kDa. The apparent molecular mass of the recombinant protein is approximately 47 and 45 kDa in SDS-PAGE under reducing conditions due to glycosylation.

(41.7+54.1) % as determined by SDS-PAGE

Measured by its binding ability in a functional ELISA.
Immobilized rat IL12B (cat: 503752) at 10 μg/ml (100 μl/well) can bind biotinylated mouse IL12RB2 (Cat:502566). The EC₅₀ of biotinylated mouse IL12RB2 (Cat:502566) is 0.6-1.5 μg/ml.