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Product Name
Anti-IL12B/IL-12B antibody
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Description
Rabbit polyclonal to IL12B/IL-12B
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Tested applications
ELISA, IHC-P
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Species reactivity
Rat IL12B / IL-12B
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Alternative names
CLMF antibody; CLMF2 antibody; Il12b antibody; IL12B antibody; IL-12B antibody; Il-12b antibody; Il12p40 antibody; Il-12p40 antibody; NKSF antibody; NKSF2 antibody; RP23-388G23.1 antibody; IMD28 antibody; CLMF antibody; p40 antibody; Il-12b antibody; Il12p40 antibody; Il-12p40 antibody; NKSF antibody; CLMF2 antibody; IMD29 antibody; NKSF2 antibody; IL-12B antibody; P40 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Rat IL12B / IL-12B (rh IL12B / IL-12B; G3V9Y5; Met1-Ser335). IL12B / IL-12B specific IgG was purified by Rat IL12B / IL-12B affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 5-10 μg/ml
ELISA:0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Rat IL12B / IL-12B. The detection limit for Rat IL12B / IL-12B is < 0.039 ng/well.
IHC-P: 0.1-2 μg/ml
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Validations
IL12B / IL-12B Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
IL12B / IL-12B Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
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Background
Subunit beta of interleukin 12 (also known as natural killer cell stimulatory factor 2, or cytotoxic lymphocyte maturation factor 2, p40) (IL12B) is a subunit of human interleukin 12. IL12B/IL-12B is a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. Interleukin 12 is a disulfide-linked heterodimer composed of the 40 kD cytokine receptor like subunit encoded by this gene, and a 35 kD subunit encoded by IL12A. IL12B/IL-12B is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. This cytokine has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Overexpression of this gene was observed in the central nervous system of patients with multiple sclerosis (MS), suggesting a role of this cytokine in the pathogenesis of the disease. The promoter polymorphism of this gene has been reported to be associated with the severity of atopic and non-atopic asthma in children. IL12B/IL-12B associates with IL23A to form the IL-23 interleukin, an heterodimeric cytokine which functions in innate and adaptive immunity.
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References
- Taoufik Y, et al. (1997) Human immunodeficiency virus gp120 inhibits interleukin-12 secretion by human monocytes: an indirect interleukin-10-mediated effect. Blood. 89 (8): 2842-8.
- Fantuzzi L, et al. (1996) Induction of interleukin-12 (IL-12) by recombinant glycoprotein gp120 of human immunodeficiency virus type 1 in human monocytes/macrophages: requirement of gamma interferon for IL-12 secretion. J Virol. 70 (6): 4121-4.
- Aragane Y, et al. (1995) IL-12 is expressed and released by human keratinocytes and epidermoid carcinoma cell lines. J Immunol. 153 (12): 5366-72.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"