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Product Name
Mouse IL12B/IL-12B recombinant protein
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Description
Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.
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Protein name
Interleukin-12 subunit beta
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Protein short names
IL12B; IMD28; P40; NKSF; CLMF; CLMF2; RP23-388G23.1; IL12P40; IL-12P40; NKSF2; IL-12B; IMD29
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Uniprot ID
P43432
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Gene Name
Il12b
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Source/Expression Host
Human Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the mouse IL12B (P43432) (Met 1-Ser 335) was expressed with six amino acids (LEVLFQ) at the C-terminus.
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Protein Species
Mouse
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Molecular weight
The recombinant mouse IL12B consists of 320 amino acids and has a calculated molecular mass of 36.6 kDa. The recombinant protein migrates as an approximately 46 kDa band in SDS-PAGE under reducing conditions.
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Purity
> 85 % as determined by SDS-PAGE
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Activity
1. Measured by its binding ability in a functional ELISA.
2. Immobilized mouse IL12B at 10μg/mL(100μL/well) can bind biotinylated human IL12RB1-His (Cat:501974).
The EC50 of biotinylated human IL12RB1-His (Cat:501974) is 0.11-0.25μg/mL. -
Validations
Mouse IL12B / IL-12B Protein SDS-PAGE
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