Mouse ICAM-1/CD54 (His Tag) recombinant protein

Mouse ICAM-1/CD54 (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Intercellular adhesion molecule-1 (ICAM-1, or CD54) is a 90 kDa member of the immunoglobulin (Ig) superfamily and is critical for the firm arrest and transmigration of leukocytes out of blood vessels and into tissues. ICAM-1 is constitutively present on endothelial cells, but its expression is increased by proinflammatory cytokines. The endothelial expression of ICAM-1 is increased in atherosclerotic and transplant-associated atherosclerotic tissue and in animal models of atherosclerosis. Additionally, ICAM-1 has been implicated in the progression of autoimmune diseases. ICAM-1 is a ligand for LFA-1(integrin). When activated, leukocytes bind to endothelial cells via ICAM-1/LFA-1 interaction and then transmigrate into tissues. Presence with heavy glycosylation and other structural characteristics, ICAM-1 possesses binding sites for a number of immune-associated ligands and serves as the binding site for entry of the major group of human Rhinovirus (HRV) into various cell types. ICAM-1 also becomes known for its affinity for Plasmodium falciparum-infected erythrocytes (PFIE), providing more of a role in infectious disease. Previous studies have shown that ICAM-1 is involved in inflammatory reactions and that a defect in ICAM-1 gene inhibits allergic contact hypersensitivity.

Intercellular adhesion molecule 1

ICAM-1; BB2; MGC6195; ICAM1; LY-47; CD54; P3.58; MYD10; MALA-2

Q3U8M7

Icam1; Icam-1

Human Cells

A DNA sequence encoding the extracellular domain of mouse ICAM1 (NP_034623.1) (Met 1-Asn 485) was fused with a polyhistidine tag at the C-terminus.

Mouse

The recombinant mouse ICAM1 consists of 469 amino acids after removal of the signal peptide. and has a predicted molecular mass of 51.7 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm ICAM1 is approximately 80-90 kDa due to glycosylation.

> 96 % as determined by SDS-PAGE

Measured by the ability of the immobilized protein to support the adhesion of PMA stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells.
When cells are added to mouse ICAM1 coated plates (12.5 μg/mL, 100 μL/well), approximately > 40% cells will adhere specifically.