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Product Name
Anti-ICAM1 (4F5) Mouse antibody
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Description
ICAM1 (4F5) Mouse monoclonal antibody
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Tested applications
WB, ICC/IF
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Species reactivity
Human
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Alternative names
Antigen identified by monoclonal;BB2;BB 2;BB2;CD 54;CD_antigen=CD54;CD54;CD54 antigen; Cell surface glycoprotein P3.58;Human rhinovirus receptor;ICAM 1;ICAM-1;ICAM1; ICAM1_HUMAN;Intercellular adhesion molecule 1;intercellular adhesion molecule 1 (CD54) antibody; h antibody
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Isotype
Mouse IgG2b
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Preparation
Antigen: Purified recombinant human CD54(ICAM-1) protein fragments expressed in E.coli.
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Clonality
Monoclonal
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Formulation
Purified mouse monoclonal in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/1000
;ICC: 1/200
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Validations
Immunocytochemistry staining of SW480 cells fixed by anhydrous methanol for 2 h at -20u2103 and using anti-CD54(ICAM-1) mouse mAb (dilution 1:200).
Western blot detection of CD54(ICAM-1) in SW480 and Raji cell lysates using CD54(ICAM-1) mouse mAb (1:1000 diluted).Predicted band size:96KDa.Observed band size:96KDa.
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Background
Swiss-Prot Acc.P05362.ICAM1 a type I membrane protein of the immunoglobulin superfamily. Is a ligand for the leukocyte adhesion LFA-1 protein (Integrin alpha-L/beta-2) and a Rhinovirus receptor. Typically expressed on endothelial cells and cells of the immune system.
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