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Product Name
Mouse ICAM-1/CD54 (His & Fc Tag) recombinant protein
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Description
ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation (By similarity).
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Protein name
Intercellular adhesion molecule 1
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Protein short names
BB2; ICAM-1; ICAM1; MGC6195; LY-47; CD54; P3.58; MYD10; MALA-2
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Uniprot ID
Q3U8M7
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Gene Name
Icam1; Icam-1
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Source/Expression Host
Human Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the extracellular domain of mouse ICAM1 (NP_034623.1) (Met 1-Asn 485) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
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Protein Species
Mouse
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Molecular weight
The recombinant mouse ICAM1/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer consists of 706 amino acids and has a predicted molecular mass of 78.3 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm ICAM1/Fc monomer is approximately 120-130 kDa due to glycosylation.
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Purity
> 96 % as determined by SDS-PAGE
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Activity
Measured by the ability of the immobilized protein to support the adhesion of PMA stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells. When cells are added to mouse ICAM1 coated plates (12.5 μg/ml, 100 μl/well), approximately >50% cells will adhere specifically.
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Validations
Mouse ICAM-1 / CD54 Protein (His & Fc Tag) SDS-PAGE
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