-
Product Name
Rat ICAM-1/CD54 (His Tag) recombinant protein
- Documents
-
Description
ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation (By similarity).
-
Protein name
Intercellular adhesion molecule 1
-
Protein short names
MYD10; MALA-2; P3.58; CD54; LY-47; ICAM1; MGC6195; BB2; ICAM-1
-
Uniprot ID
Q00238
-
Gene Name
Icam1; Icam-1
-
Source/Expression Host
Human Cells
-
Expression Plasmid/cDNA
A DNA sequence encoding the rat ICAM1 (Q00238-1) extracellular domain (Met 1-Thr 493) was expressed, fused with a polyhistidine tag at the C-terminus.
-
Protein Species
Rat
-
Molecular weight
The recombinant rat ICAM1 comprises 477 amino acids and predicts a molecular mass of 52.7 kDa. The apparent molecular mass of the rat ICAM1 is approximately 70-75 kDa in SDS-PAGE under reducing conditions due to glycosylation.
-
Purity
> 97 % as determined by SDS-PAGE
-
Activity
Measured by the ability of the immobilized protein to support the adhesion of PMA stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells.
When cells are added to rat ICAM1 coated plates (12.5 µg/mL, 100 µL/well), approximately > 30% cells will adhere specifically. -
Validations
Rat ICAM-1 / CD54 Protein (His Tag) SDS-PAGE
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"