Human IL-1 beta recombinant protein

Human IL-1 beta recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Interleukin-1 beta (IL1 beta or IL1B) also known as catabolin, is a member of the interleukin 1 cytokine family. IL1 is a pleiotropic cytokine. It is involved in the inflammatory response, cell growth, and tissue repair in the cortex. The IL1 superfamily consists of three members, IL1A (IL1 alpha), IL1B (IL1 beta), and IL1 receptor antagonist (IL1Ra). In clinical, it has been reported that Interleukin (IL)-1 may influence Th1 / Th2 immune responsiveness and has been implicated in the establishment of successful pregnancy. Proinflammatory interleukin (IL)-1 gene polymorphisms associated with high levels of IL-1beta activity increase the risk for hypochlorhydria and distal gastric carcinoma. IL1B polymorphisms may be involved in susceptibility to SSc. Moreover, the IL2-384-G allele may be a marker for the limited phenotype of systemic sclerosis (SSc).

Interleukin-1 beta

IL-1; IL-1 BETA; IL1F2; IL-1B; IL-1BETA; IL1B; IL1-BETA

P01584

IL1B; IL1F2

E. coli

A DNA sequence encoding the mature form of human IL1? (NP_000567.1) (Ala 117-Ser 269) was expressed.

Human

The recombinant human IL1? consisting of 154 amino acids migrates with an apparent molecular mass of 17 kDa as estimated in SDS-PAGE under reducing conditions.

> 95 % as determined by SDS-PAGE

1. Measured by its binding ability in a functional ELISA. Immobilized recombinant human IL1B at 2 μg/mL (100μl/well) can bind recombinant human IL1R2 with a linear range of 6.25-400 ng/ml.
2. Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED₅₀ for this effect is typically 2-8 pg/mL.
3. Measured by its binding ability in a functional ELISA.
4. Immobilized human IL1B at 10 μg/mL (100 μl/well) can bind human IL1R1, The EC₅₀ of human IL1R1 is 0.48 μg/mL.