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Product Name
Anti-IL-1 beta Rabbit antibody
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Description
IL-1 beta Rabbit polyclonal antibody
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Tested applications
WB
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Species reactivity
Human, Mouse, Rat
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 124-223 of human IL1B (NP_000567.1).CTLRDSQQKSLVMSGPYELKALHLQGQDMEQQVVFSMSFVQGEESNDKIPVALGLKEKNLYLSCVLKDDKPTLQLESVDPKNYPKKKMEKRFVFNKIEIN
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide,50% glycerol,pH7.3.
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Storage instructions
Store at -20°C . Avoid freeze / thaw cycles.
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Applications
WB: 1/500 - 1/2000
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Validations
Western blot - IL1B Polyclonal Antibody. Western blot analysis of extracts of various cell lines, using IL1B antibody .Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
Western blot - IL1B Polyclonal Antibody. Western blot analysis of extracts of various cell lines, using IL1B antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Exposure time: 10s.
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Background
Swiss-Prot Acc.P01584.The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.
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