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Product Name
Anti-IL-1 beta antibody
- Documents
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Description
Mouse monoclonal to IL-1 beta
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Tested applications
ELISA, IHC-P
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Species reactivity
Human IL-1 beta / IL1B
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Alternative names
IL-1 antibody; IL-1 beta antibody; IL1B antibody; IL1-BETA antibody; IL1F2 antibody; IL-1 antibody; IL1F2 antibody; IL1-BETA antibody; Il-1b antibody; IL-1beta antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human IL-1 beta / IL1B (rh IL-1 beta / IL1B; NP_000567.1; Met1-Ser269). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
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Clonality
Monoclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
ELISA: 0.5-1 μg/mL
This antibody can be used at 0.5-1 μg/ml with the appropriate secondary reagents to detect Human IL-1 beta.
IHC-P: 1-10 μg/mL
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Validations
IL-1 beta / IL1B Antibody, Mouse MAb, Immunohistochemistry
Immunochemical staining of human IL1b in human liver (from 2 donors) with mouse monoclonal antibody (5 µg/mL, formalin-fixed paraffin embedded sections).
IL-1 beta / IL1B Antibody, Mouse MAb, Immunohistochemistry
Immunochemical staining of human IL1b in human lung with mouse monoclonal antibody (5 µg/mL, formalin-fixed paraffin embedded sections).
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Background
Interleukin-1 beta (IL1 beta or IL1B) also known as catabolin, is a member of the interleukin 1 cytokine family. IL1 is a pleiotropic cytokine. It is involved in the inflammatory response, cell growth, and tissue repair in the cortex. The IL1 superfamily consists of three members, IL1A (IL1 alpha), IL1B (IL1 beta), and IL1 receptor antagonist (IL1Ra). In clinical, it has been reported that Interleukin (IL)-1 may influence Th1 / Th2 immune responsiveness and has been implicated in the establishment of successful pregnancy. Proinflammatory interleukin (IL)-1 gene polymorphisms associated with high levels of IL-1beta activity increase the risk for hypochlorhydria and distal gastric carcinoma. IL1B polymorphisms may be involved in susceptibility to SSc. Moreover, the IL2-384-G allele may be a marker for the limited phenotype of systemic sclerosis (SSc).
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References
- Kim SH, et al. (2008) Association of -31TC and -511 CT polymorphisms in the interleukin 1 beta (IL1B) promoter in Korean keratoconus patients. Mol Vis. 14:2109-16.
- Wang ZC, et al. (2002) T helper 1-type immunity to trophoblast antigens in women with a history of recurrent pregnancy loss is associated with polymorphism of the IL1B promoter region. Genes Immun. 3(1): 38-42.
- Mattuzzi S, et al. (2007) Association of polymorphisms in the IL1B and IL2 genes with susceptibility and severity of systemic sclerosis. J Rheumatol. 34(5): 997-1004.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"