Human HER2/ErbB2 (His & GST Tag) recombinant protein

Human HER2/ErbB2 (His & GST Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Human epidermal growth factor receptor 2 (HER2), also known as ErbB2, NEU, and CD340, is a type I membrane glycoprotein, and belongs to the epidermal growth factor (EGF) receptor family. HER2 protein cannot bind growth factors due to the lacking of ligand binding domain of its own and autoinhibited constitutively. However, HER2 forms a heterodimer with other ligand-bound EGF receptor family members, therefore stabilizes ligand binding and enhances kinase-mediated activation of downstream molecules. HER2 plays a key role in development, cell proliferation and differentiation. HER2 gene has been reported to associate with malignancy and a poor prognosis in numerous carcinomas, including breast, prostate, ovarian, lung cancers and so on.

Receptor tyrosine-protein kinase erbB-2

C-ERBB-2; ERBB2; C-NEU; HER-2; RP23-355L10.8; NEU; MKIAA3023; CD340; NGL; HER2; HER-2/NEU; ERBB-2; TKR1; C-ERBB2; MLN19

X5DNK3

ERBB2; HER2; MLN19; NEU; NGL

Baculovirus-Insect Cells

A DNA sequence encoding the cytoplasmic domain (Lys 676-Val 1255) of human ErbB2 (NP_004439.2) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Human

The recombinant human ErbB2 (676-1255)/GST chimera consists of 817 amino acids and predictes a molecular mass of 92 kDa. It migrates as an approximately 100 KDa band in SDS-PAGE under reducing conditions.

> 75 % as determined by SDS-PAGE

No Kinase Activity