Human caspase-3 / CASP-3 (His Tag) recombinant protein

Human caspase-3 / CASP-3 (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage (By similarity).

Caspase-3

CASP-3; PARP CLEAVAGE PROTEASE; CPP32; CYSTEINE PROTEASE CPP32; SCA-1; MLDY; APOPTOSIS RELATED CYSTEINE PROTEASE; AC-3; CASPASE 3; YAMA; APOPTOSIS-RELATED CYSTEINE PEPTIDASE; CASPASE-3; CPP32B; CASPASE-3; PROCASPASE3; A830040C14RIK; PROTEIN YAMA; CPP-32; LICE; APOPAIN; CC3; CASP3; SREBP CLEAVAGE ACTIVITY 1

Q5IS54

CASP3

E. coli

A DNA sequence encoding the mature form of human caspase3 (Q5IS54) (Ser29-His277) was expressed with a polyhistidine tag at the C-terminus.

Human

The recombinant human caspase3 consists of 259 amino acids and predicts a molecular mass of 29.9 KDa. It migrates as an approximately 17 and 15 KDa band in SDS-PAGE under reducing conditions.

(48.4+48.1) % as determined by SDS-PAGE

Measured by its ability to cleave the fluorogenic peptide substrate Ac-DEVD-AFC.
The specific activity is > 5000 pmoles/min/μg.