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Product Name
BCL2 antibody
- Documents
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Description
BCL2 Mouse Monoclonal antibody. Positive IHC detected in human tonsillitis tissue, human lymphoma tissue. Positive FC detected in Jurkat cells. Positive IP detected in U-937 cells. Positive WB detected in Jurkat cells, U-937 cells. Observed molecular weight by Western-blot: 26 kDa
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Tested applications
ELISA, FC, IP, IHC, WB
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Species reactivity
Human; other species not tested.
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Alternative names
Apoptosis regulator Bcl 2 antibody; B cell CLL/lymphoma 2 antibody; BCL2 antibody; Oncogene B cell leukemia 2 antibody
- Immunogen
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Isotype
Mouse IgG2b
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Preparation
This antibody was obtained by immunization of BCL2 recombinant protein (Accession Number: XM_047437733). Purification method: Caprylic acid/ammonium sulfate precipitation.
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Clonality
Monoclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:500-1:5000
IHC: 1:100-1:2000
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:107080(BCL2 Antibody) at dilution of 1:4000
Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using Catalog No:107080(BCL2 Antibody) at dilution of 1:1000 (under 10x lens)
Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using Catalog No:107080(BCL2 Antibody) at dilution of 1:1000 (under 40x lens)
1X10^6 Jurkat cells were stained with 0.2ug BCL2 antibody (Catalog No:107080, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000.
IP Result of anti-BCL2 (IP:Catalog No:107080, 5ug; Detection:Catalog No:107080 1:1000) with U-937 cells lysate 4000ug.
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Background
BCL2 belongs to the Bcl-2 family. It suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. BCL2 regulates cell death by controlling the mitochondrial membrane permeability. It appears to function in a feedback loop system with caspases. BCL2 inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).
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References
- Zeng J, Dai P, Ren L. Apoptosis-induced anti-tumor effect of Curcuma kwangsiensis polysaccharides against human nasopharyngeal carcinoma cells. Carbohydrate polymers. 89(4):1067-72. 2012.
- Liu C, Hong T, Shao M, Chen Z, Wang C. Melatonin synergized with cyclosporine A improves cardiac allograft survival by suppressing inflammation and apoptosis. Molecular medicine reports. 10(3):1323-8. 2014.
- Sun Y, Liu Z, Zou X. Mechanisms underlying 3-bromopyruvate-induced cell death in colon cancer. Journal of bioenergetics and biomembranes. 47(4):319-29. 2015.
- Duan X, Wang W, Liu X, Yan H, Dai R, Lin Q. Neuroprotective effect of ethyl acetate extract from gastrodia elata against transient focal cerebral ischemia in rats induced by middle cerebral artery occlusion. Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan / sponsored by All-China Association of Traditional Chinese Medicine, Academy of Traditional Chinese Medicine. 35(6):671-8. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"