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Anti-Bcl-2 (3H5) Mouse antibody

Cat.#: 168157

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Product Information

  • Product Name
    Anti-Bcl-2 (3H5) Mouse antibody
  • Documents
  • Description
    Bcl-2 (3H5) Mouse monoclonal antibody
  • Tested applications
    ICC/IF, WB, IHC-P
  • Species reactivity
    Human, Mouse, Rat, Chicken
  • Isotype
    Mouse IgG1
  • Preparation
    Antigen: Synthetic Peptide of Bcl-2
  • Clonality
    Monoclonal
  • Formulation
    PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
  • Storage instructions
    Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Applications

    IF: 1/50-200

    WB: 1/1000-2000

    IHC: 1/200

  • Validations

    Western blot detection of Bcl-2 in K562,Jurkat cell lysates using Bcl-2 (3H5) Mouse mAb(1:1000 diluted).Predicted band size:26KDa.Observed band size:26KDa.

    Western blot detection of Bcl-2 in K562,Jurkat cell lysates using Bcl-2 (3H5) Mouse mAb(1:1000 diluted).Predicted band size:26KDa.Observed band size:26KDa.

    Western blot detection of Bcl-2 in human breast cancer cell line MCF-7(A), MDA-MB-231(B) and Cal51(C) using Bcl-2 mouse mAb (YM3041, 1:2000 diluted).Predicted band size: 26kDa.Observed band size:26kDa. Picture was kindly provided by our customer from Tianjin Medical University Cancer Institute and Hospital

    Western blot detection of Bcl-2 in human breast cancer cell line MCF-7(A), MDA-MB-231(B) and Cal51(C) using Bcl-2 mouse mAb (YM3041, 1:2000 diluted).Predicted band size: 26kDa.Observed band size:26kDa. Picture was kindly provided by our customer from Tianjin Medical University Cancer Institute and Hospital

    Western Blot analysis of chicken cell lysis using Antibody diluted at 1:1000

    Western Blot analysis of chicken cell lysis using Antibody diluted at 1:1000

    Western blot analysis of Hela, diluted at 1:1000

    Western blot analysis of Hela, diluted at 1:1000

    The picture was kindly provided by our customer

    The picture was kindly provided by our customer

    Immunohistochemical analysis of paraffin-embedded Human breast cancer. 1, Using Bcl-2 Mouse mAb (YM3041) diluted at 1:200 (4°,overnight). 2, High-pressure and temperature Citric acid, pH6.0 was used for anbitody retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 50min). Picture was kindly provided by our customer from Tianjin Medical University Cancer Institute and Hospital

    Immunohistochemical analysis of paraffin-embedded Human breast cancer. 1, Using Bcl-2 Mouse mAb (YM3041) diluted at 1:200 (4°,overnight). 2, High-pressure and temperature Citric acid, pH6.0 was used for anbitody retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 50min). Picture was kindly provided by our customer from Tianjin Medical University Cancer Institute and Hospital

    IHC staining of Human tonsil tissue paraffin-embedded, diluted at 1:200.

    IHC staining of Human tonsil tissue paraffin-embedded, diluted at 1:200.

  • Background
    Swiss-Prot Acc.P10415.Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785).
  • References
    • Aqueous extract of Fritillariae cirrhosae induces cellular apoptosis through activation of STATs-mediated immunomodulation

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"