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Product Name
Anti-Bcl-2 Rabbit antibody
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Description
Bcl-2 Rabbit polyclonal antibody
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Tested applications
WB, IHC-P, ICC/IF, FC
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Species reactivity
Human, Mouse
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthesized peptide derived from human Bcl-2
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Clonality
Polyclonal
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Formulation
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/1000-1/2000
IHC: 1/50-1/200
ICC/IF: 1/50-1/200
FC: 1/50
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Validations
Western blot analysis of extracts from 293T, 3T3 and Hela cells using Bcl-2 Rabbit pAb at 1:1000 dilution. Predicted band size: 26kDa. Observed band size: 26kDa.
Immunocytochemistry staining of MCF-7 cells (green) fixed with paraformaldehyde, permeabilised with 0.25% Triton X100/PBS, and using Bcl-2 Antibody. The nuclear counter stain is DAPI (blue).
Immunohistochemical analysis of paraffin-embedded human tonsil, using Bcl-2 Antibody.
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Background
Swiss-Prot Acc.P10415.This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends.
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References
- Nrf2 protects against acute lung injury and inflammation by modulating TLR4 and Akt signaling.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"