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BCL2 antibody

Cat.#: 108391

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Product Information

  • Product Name
    BCL2 antibody
  • Documents
  • Description
    BCL2 Rabbit Polyclonal antibody. Positive IP detected in MCF-7 cells, HepG2 cells, HL-60 cells. Positive WB detected in HL-60 cells, HepG2 cells, Jurkat cells, mouse spleen tissue, rat brain tissue, rat spleen tissue, RAW 264.7 cells, U-937 cells. Positive IHC detected in human tonsillitis tissue, human breast cancer tissue, human colon cancer tissue, human lung cancer tissue, human lymphoma tissue. Positive FC detected in Jurkat cells. Observed molecular weight by Western-blot: 25-30 kDa
  • Tested applications
    ELISA, WB, IP, IHC, FC
  • Species reactivity
    Human, Mouse, Rat; other species not tested.
  • Alternative names
    Apoptosis regulator Bcl 2 antibody; B cell CLL/lymphoma 2 antibody; BCL2 antibody; Oncogene B cell leukemia 2 antibody
  • Immunogen
    Human BCL2 Recombinant protein (GST tag)
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of BCL2 recombinant protein (Accession Number: XM_047437733). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:500-1:5000

    IHC: 1:20-1:200

  • Validations

    HL-60 cells were subjected to SDS PAGE followed by western blot with Catalog No:108391(BCL2 antibody) at dilution of 1:1000

    HL-60 cells were subjected to SDS PAGE followed by western blot with Catalog No:108391(BCL2 antibody) at dilution of 1:1000

    Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using Catalog No:108391(BCL2 Antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using Catalog No:108391(BCL2 Antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using Catalog No:108391(BCL2 Antibody) at dilution of 1:50 (under 40x lens)

    Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using Catalog No:108391(BCL2 Antibody) at dilution of 1:50 (under 40x lens)

    IP Result of anti-BCL2 (IP:Catalog No:108391, 3ug; Detection:Catalog No:108391 1:1000) with MCF-7 cells lysate 2500ug.

    IP Result of anti-BCL2 (IP:Catalog No:108391, 3ug; Detection:Catalog No:108391 1:1000) with MCF-7 cells lysate 2500ug.

    1X10^6 Jurkat cells were stained with 0.2ug BCL2 antibody (Catalog No:108391, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

    1X10^6 Jurkat cells were stained with 0.2ug BCL2 antibody (Catalog No:108391, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

  • Background
    BCL2 belongs to the Bcl-2 family. It suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. BCL2 regulates cell death by controlling the mitochondrial membrane permeability (PMID: 10365962). It appears to function in a feedback loop system with caspases. BCL2 inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1) (PMID: 10444588).
  • References
    • Liu H, Liu J, Xu L, Zhou S, Li L, Liu W. Enantioselective cytotoxicity of isocarbophos is mediated by oxidative stress-induced JNK activation in human hepatocytes. Toxicology. 276(2):115-21. 2010.
    • Chen W, Hou J, Yin Y. alpha-Bisabolol induces dose- and time-dependent apoptosis in HepG2 cells via a Fas- and mitochondrial-related pathway, involves p53 and NFkappaB. Biochemical pharmacology. 80(2):247-54. 2010.
    • Zhang J, Guo H, Zhang H. Putative tumor suppressor miR-145 inhibits colon cancer cell growth by targeting oncogene Friend leukemia virus integration 1 gene. Cancer. 117(1):86-95. 2011.
    • Yu JQ, Yin Y, Lei JC. Activation of apoptosis by ethyl acetate fraction of ethanol extract of Dianthus superbus in HepG2 cell line. Cancer epidemiology. 36(1):e40-5. 2012.
    • Yin Y, Chen W, Tang C. NF-κB, JNK and p53 pathways are involved in tubeimoside-1-induced apoptosis in HepG2 cells with oxidative stress and G₂/M cell cycle arrest. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association. 49(12):3046-54. 2011.
    • Ding F, Shao ZW, Yang SH, Wu Q, Gao F, Xiong LM. Role of mitochondrial pathway in compression-induced apoptosis of nucleus pulposus cells. Apoptosis : an international journal on programmed cell death. 17(6):579-90. 2012.
    • Liu W, Zhang Y, Hao J. Nestin protects mouse podocytes against high glucose-induced apoptosis by a Cdk5-dependent mechanism. Journal of cellular biochemistry. 113(10):3186-96. 2012.
    • Lei JC, Yu JQ, Yin Y, Liu YW, Zou GL. Alantolactone induces activation of apoptosis in human hepatoma cells. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association. 50(9):3313-9. 2012.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"