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Product Name
Anti-Bcl-2 Rabbit antibody
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Description
Bcl-2 Rabbit polyclonal antibody
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Tested applications
WB, IHC-P, ICC/IF, FC
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Species reactivity
Human, Mouse
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Alternative names
Bcl-2; PPP1R50 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant protein of human Bcl-2
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Clonality
Polyclonal
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Formulation
Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Storage instructions
Store at -20°C. Stable for 12 months from date of receipt.
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Applications
WB: 1/1000
IHC: 1/20-1/500
ICC/IF: 1/20
FC: 1/50
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Validations
Western blot detection of Bcl-2 in MCF-7 cell lysates using Bcl-2 Rabbit pAb(1:1000 diluted).Predicted band size:26KDa.Observed band size:26KDa.
Western blot detection of Bcl-2 in K562,CHO-K1,3T3,Hela cell lysates using Bcl-2 Rabbit pAb(1:1000 diluted).Predicted band size:26KDa.Observed band size:26KDa.
Immunohistochemistry of Bcl-2 in paraffin-embedded Human breast cancer tissue using Bcl-2 Rabbit pAb at dilution 1/100
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Background
Swiss-Prot Acc.P10415.Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"